Which techniques should be used to screen blood donations for hepatitis B surface antigen?

Which techniques should be used to screen blood donations for hepatitis B surface antigen?

Preservation of all donor sera already tested for Hepatitis B surface antigen (HBsAg) by counterimmunoelectrophoresis (C.I.E.P.) allowed C.I.E.P., reversed passive haemagglutination (R.P.H.A.), and radioimmunoassay (R.I.A.) to be evaluated as screening techniques. Out of 165 811 donors tested for the first time 207 were found by C.I.E.P. to be positive for HBsAf-a prevalence of 0-12%. At the ne...

Hepatitis Be antigen and antibody in hepatitis B surface antigen positive blood donors.

In a study of 105 asymptomatic HBsAg positive blood donors, 9 (8.6%) were found to have HBeAg, 38 (36.2%) anti-HBe, and the remaining 58 (55.2%) neither marker detectable by gel diffusion. There was no correlation between HBeAg/anti-HBe status and HBsAg sub-types, Glm allotypes, the presence of anti-Gm, red cell antibodies, or rheumatoid factor. Rheumatoid factor activity could be removed from ...

Which treatment option should be used?

EXPRESSION OF HEPATITIS B SURFACE ANTIGEN IN SACCHAROMYCES CEREVISIAE

The genome of HB V virus of serotype ayw cloned in pBR322 and expression shuttle vector p YES2 were used for construction of the HBsAg chimeric genes and their expression in Saccharomyces cerevisiae. Two recombinant plasmids were constructed. One of them contained the coding sequences for the major polypeptide of surface antigen. Another construct carried the major polypeptide with the pre-S2 a...

Should hepatitis B surface antigen positive mothers breast feed?

Breast fed infants may be at greater risk of mother to infant hepatitis B virus infection compared with formula fed infants. We studied 85 infants born to 84 hepatitis B surface antigen positive mothers (only two of whom were hepatitis B e positive), and who had received immunisation against hepatitis B virus. Our results indicate that breast feeding does not increase the risk of developing hep...